The aspidosperma genus apocynaceae represents one of the largest sources of indole alkaloids widely associated with cardiovascular effects. Antidiabetic potential of two medicinal plants used in gabonese folk medicine huguette agnaniet1, elvis jolinom mbot1. Complete 1 h and c nmr data of the new compound were reported. Antibacterial and antibioticresistance modifying activity. Sp are used as traditional medicines in gabon for the treatment of different diseases, especially in the case of diabetes. To prepare the anticancer indoles, a series of substituted heteroannulated indole derivatives has been synthesized and characterized by spectral and elemental analysis.
Nauclea pobeguinii are used in folk medicine for the treatment of different diseases, especially in the case of diabetes associated or not to hypertension. Nauclea pobeguinii is used in traditional medicine as abortive and for the treatment of stomach ache, infectious diseases, jaundice, fever, diarrhea, worm, and malaria. This study was initiated to screen the antimicrobial activities of methanol. A research paper, showing the presence of a natural painrelieving opoid in the bark and roots of nauclea latifolia. Antimalarial activity and toxicity evaluation of a quantified nauclea pobeguinii extract article in journal of ethnopharmacology 11.
Extracts of the plant have exhibited activity against escherichia coli, shigella flexneri, salmonella typhi and staphylococcus aureus which are responsible for gastroenteritis in children. Nauclea is a genus of flowering plants in the family rubiaceae. Antimalarial efficacy of a quantified extract of nauclea. Natural products as starting points for future antimalarial therapies. Antimalarial activity and toxicity evaluation of a quantified. Ethnobotanical survey on medicinal plants used by guinean. Eastern and western africa medicinal plants for cancer therapy. Syzygium jambos displayed antibacterial and antibiotic. Antimalarial efficacy of a quantified extract of nauclea pobeguinii stem bark in human adult volunteers with diagnosed uncomplicated falciparum malaria. These results justify the use of these plants in traditional medicine and the practice of supplementing decoctionsconcoctions with conventional antibiotics. The terminal vegetative buds are usually strongly flattened.
Identification of strictosamide, 8 known iridoidindole alkaloids, and a phenol glycoside. The control of these infections is increasingly complicated due to development of resistance against available drugs. The aim of this study was to evaluate the antidiabetic potential of. Petit rubiaceae, a plant used in traditional medicine in dr congo against malaria. A new method was developed and validated for the quantification of strictosamide in the extract of the stem bark of nauclea pobeguinii. Diabetes mellitus is a metabolic disorder which is rising globally in rich and developing countries. There are other feverreducing folk remedies that could yield a nextgeneration antimalarial. The total 80% etoh extract of stem bark of nauclea pobeguinii rubiaceae, was analysed by means of uplcms and hplcspenmr. Among gabonese medicinal plants, sarcocephalus pobeguinii hua ex pellegr. In this study, a new apoptotic monoterpenoid indole alkaloid, subditine 1, and four known compounds were isolated from the bark of nauclea subdita. Antimalarial activity and toxicity evaluation of a. Extracts from nauclea pobeguinii are active in both rodent models and patients.
The leaves of nauclea pobeguinii are used for the treatment of malaria in the dr congo while the stem bark is used for the prevention of threatened abortion in upper nyong valley in cameroon. Recently, the plant was shown to have cytotoxic effects on various hematological and carcinoma cell lines. Nauclea pobeguinii and uapaca togoensis has the potential to fight against multidrug resistant mdr gram negative bacteria and cancer cells lines. Its antiplasmodial activity has been reported before.
By definition, antioxidants are complex compounds found in our diet that act as a protective shield for our. Nov 04, 2014 this page was last edited on 20 december 2019, at 14. Phytochemical analysis with free radical scavenging, nitric. Natural products as starting points for future anti. Nauclea latifolia extract in hot water, cold water, petroleum ether and chloroform at concentrations of 200, 150, 100, 50% were tested on some pathogenic bacteria such as staphylococcus aureus, escherichia coli, salmonella typhi and pseudomonas aeruginosa. The present study was designed to evaluate the antibacterial activities of methanol extracts of bark and leaves of syzygium jambos, as well as their synergistic effects with selected antibiotics against drugresistant grampositive and gramnegative bacteria. Considering the promising results of phase i clinical trials with herbal medicine cobaty017, a phase ii study was conducted with plasmodium falciparum malariainfected patients, for efficacy and safety evaluation of cobaty017 compared with artemetherlumefantrine used as a positive control. Traditional uses, phytochemistry and pharmacological. Sep 07, 2010 it suggested that flavonoids and saponins were responsible for the antipyretic and antinociceptive effects of nauclea latifolia roots decoction. Mesia k1, tona l, mampunza mm, ntamabyaliro n, muanda t, muyembe t, musuamba t, mets t, cimanga k, totte j, pieters l, vlietinck aj. The structures of all isolated compounds were elucidated with various spectroscopic methods such as 1d and 2d nmr, ir, uv and lcmsittof. The hymenodicteaenaucleeae clade is a predominantly paleotropical group with 220 species in 28 genera.
The lowest ic50 value was obtained with the bark extract of n. The genus nauclea in africa comprises seven species. The hydromethanolic extract from nauclea diderrichii was the most clastogenicaneugenic fraction with a minimal active concentration mac of 23. Characterization of flowering locus t1 ft1 gene in brachypodium and wheat.
Nigerian medicinal plants are known to contain a large variety of chemical structures and some of the plant extracts have been screened for antitrypanosomal activity, in the search for potential new drugs against the illness. Pdf traditional uses, phytochemistry and pharmacological. Validated liquid chromatography mass spectrometry method. Nauclea orientalis is a species of tree in the rubiaceae family, native to southeast asia, new guinea, and australia. Nauclea diderrichii is found in angola, cameroon, central african republic, the republic of the congo, the democratic republic of the congo, ivory coast, gabon, ghana. Aspidosperma fendleri, a plant found mainly in venezuela, has a single phytochemical report in which is revealed the presence of alkaloids in its seeds. Files are available under licenses specified on their description page. For example, the bark of the plant nauclea pobeguinii, from the democratic republic of congo, can substantially reduce parasitemia in mice infected with rodent malaria 97 and has shown efficacy in man in phase iib clinical trials 98. It has scented orange flowers in dense globeshaped clusters. The aim of this phase iia clinical trial was to assess the efficacy of an 80 % ethanolic quantified extract containing 5. Despite a noticeable progress in the treatment of diabetes mellitus by synthetic drugs, the search for new natural antidiabetic agents is going on. Antimalarial activities and toxicities of three plants used. New indole alkaloids from the bark of nauclea orientalis.
Multidrug resistance of gramnegative bacteria constitutes a major obstacle in the antibacterial fight worldwide. Nauclea pobeguinii e30, the most active and synergistic of all these extracts, and some hydrolates with antimicrobial activity need further exploration for the development of novel. The discovery of new and effective antimicrobials andor resistance modulators is necessary to combat the spread of resistance or to reverse the multidrug resistance. In gabon, the species nauclea diderrichii and sarcocephalus pobeguinii syn. Jun 01, 2012 the study showed a significant decreased parasitaemia in patients treated with pr 29 ct1 and artesunateamodiaquine with adequate clinical parasitological responses apcr at day 14 of 87. Spotlight on resistant cells and molecular targets. Antipyretic and antinociceptive effects of nauclea latifolia. Neolamarckia cadamba, with english common names burflowertree, laran, and leichhardt pine, and called kadam locally, is an evergreen, tropical tree native to south and southeast asia. We also demonstrated the antiproliferative potential of nauclea pobeguinii against drugresistant cancer cell lines.
Apart from the main constituent, strictosamide, a series of minor constituents was identified, including two new iridoidindole alkaloids, i. All structured data from the file and property namespaces is available under the creative commons cc0 license. Four new alkaloids, nauclealines a 1 and b 2 and naucleosides a 3 and b 4, together with six known compounds, strictosamide 5, vincosamide 6, pumiloside 7, kelampayoside a, sitosterol, and sitosteryl. The genus name honours french naturalist jeanbaptiste lamarck. According to the promising results of the phase i and phase iia clinical trials with the herbal medicinal product pr 259 ct1 consisting of an 80 % ethanolic extract of the stem bark of nauclea pobeguinii containing 5. Full length research paper nauclea latifolia on newcastle. Full length research paper antiviral effects of nauclea latifolia on newcastle disease virus ndv onu ukamaka1, nwiyi paul2 and erumaka ikechi2 1department of microbiology and brewery, nnamdi azikiwe university, awka anambra state, nigeria. Antibacterial and antibioticresistance modifying activity of.
In this study, the leaves of terminalia ivorensis, carapa procera, fagara macrophylla, anacardium occidentale, ficus spp. Cytotoxicity of selected cameroonian medicinal plants and. The crude extracts were subjected to qualitative phytochemical screening. Growing to around 50 ft 15 m, this widely distributed tree has a conical shape and spreading branches.
African trypanosomiasis is a vectorborne parasitic disease causing serious risks to the lives of about 60 million people and 48 million cattle globally. Abstractthe antimalarial activities of crude extracts and 17 fractions from the partition of 80%methanolic extracts of three plants the stem bark of croton mubango, the stem bark of nauclea pobeguinii and the leaves of pyrenacantha staudtii used as antimalarial remedies in the democratic republic of congo were studied both in vitro against plasmodium falciparum and in mice infected with. Phylogeny and generic delimitations in the sister tribes. Pdf in vitro antiinflammatory, antioxidant and in vivo. Cytotoxicity of selected cameroonian medicinal plants and nauclea. Development and validation of an hplcmethod for the. Nauclea pobeguinii was investigated, as it was used in folk medicine. Merill rubiaceae, an indigenous hardwood of west africa, is increasingly being grown commercially. Antimalarial activity and toxicity evaluation of a quantified nauclea pobeguinii extract.
Download fullsize image highlights characterisation of the total stem bark extract of nauclea pobeguinii by uplcms and hplcspenmr. The results of this study demonstrate the cytotoxicity of six cameroonian medicinal plants, canarium schweinfurthii, dischistocalyx grandifolius, tragia benthamii, fagara macrophylla, myrianthus arboreus and nauclea pobeguinii. This page was last edited on 20 december 2019, at 14. A phase ii pilot trial to evaluate cobaty017 safety and. Phytochemical analysis with free radical scavenging. Novel indole derivatives as potential anticancer agents. In this study, we investigated the antibacterial and antibioticresistance modifying activities against 29 gram.
Phytochemical investigation of dichloromethane neutral phase of stem bark of nauclea pobeguinii led to the isolation of a new monoterpene indole. The species are evergreen trees or shrubs that are native to the paleotropics. Nauclea diderrichii is found in angola, cameroon, central african republic, the republic of the congo, the democratic republic of the congo, ivory coast, gabon, ghana, liberia, mozambique, nigeria, sierra leone, and uganda. Citations are the number of other articles citing this article, calculated by crossref and updated daily. But it is noted that active components of nauclea latifolia remain to be isolate in the further studies. Its natural habitat is subtropical or tropical moist lowland forests. Pdf nauclea pobeguinii and uapaca togoensis has the. Antibacterial and antibioticresistance modifying activity of the. The present study provides information on the possible use of nauclea pobeguinii and compound 4 in the control of gramnegative bacterial.
Antimalarial activities and toxicities of three plants. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. Cancer remains a major health hurdle worldwide and has moved from the third leading cause of death in the year 1990 to second place after cardiovascular disease since 20. Results obtained from a phase i clinical trial on healthy male. In the african region this rate is the highest, with 20 million diagnosed diabetics. It has many common names, including bur tree, canary wood, leichhardt pine and yellow cheesewood. Pdf antibacterial and antibioticresistance modifying. Antidiabetic potential of two medicinal plants used in. This study investigates the potential for vegetative propagation and clonal selection, and raises some fundamental questions about the physiology of apical dominance and of plagiotropism. Its bark is deeply furrowed and the heartshaped, deep green leaves are up. Subsequently they were evaluated for anticancer activity against cervical cancer hela cell line using mtt assay. The generic name is derived from the ancient greek words naus, meaning ship and kleio, meaning to close. Rooting ability was high, with up to 100% rooting in 24. In vitro antiinflammatory, antioxidant and in vivo antiarthritic properties of stem bark extracts from nauclea pobeguinii rubiaceae in rats.
These metrics are regularly updated to reflect usage leading up to the last few days. The present overview deals with the potential of the flora of. Text is available under the creative commons attributionsharealike license. Potential of central, eastern and western africa medicinal. Chromatographic profiling and identification of two new. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals. In addition to that of alkaloid 1, the complete cnmr data of. Hydromethanolic extracts from nauclea latifolia, nauclea diderrichii and nauclea pobeguinii exhibited a significant clastogenicaneugenic activity without s9 mix.
Compounds 5c and 5d exhibit excellent activity with the ic50 value of. The antimalarial efficacy of stem bark extract of nauclea pobeguinii in human adult volunteers with diagnosed uncomplicated falcip arum malaria was also. Antimicrobial activities of some medicinal plants against. A new indole alkaloid, naucline 1 together with four known alkaloids, angustine 2, angustidine 3, nauclefine 4 and naucletine 5, were isolated from the bark of nauclea officinalis. Subditine, a new monoterpenoid indole alkaloid from bark. Cytotoxicity of selected cameroonian medicinal plants and nauclea pobeguinii towards multifactorial drugresistant cancer cells. Antimicrobial activities of a plethora of medicinal plant. New antidiabetic and freeradical scavenging potential of. Urogenital tract infections remain a serious global treat especially in women. Read validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study, biomedical chromatography on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Read development and validation of an hplcmethod for the determination of alkaloids in the stem bark extract of nauclea pobeguinii, talanta on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Medicinal indications of these species often match the speci.
This study explored the cardiovascular effects of an ethanolic extract of a. To evaluate the in vitro and in vivo antiplasmodial activity and toxicity of the aqueous and 80% etoh extract of the stem bark of nauclea pobeguinii pob. Plants are susceptible to environmental stress and have developed numerous defense systems, resulting in formation of potent antioxidants. Chemotherapy is one of the most widely used treatment modes. Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. Naucline, a new indole alkaloid from the bark of nauclea. Nauclea diderrichii is a species of tree of the genus nauclea in the family rubiaceae. The structures of isolated compounds were elucidated with various spectroscopic methods such as 1d and 2d nmr, ir, uv and lcms. Pdf cytotoxicity of selected cameroonian medicinal. Comparative antimycobacterial activity on lowenstein. Antibacterial and antibioticresistance modifying activity of the extracts and compounds from nauclea pobeguinii against gramnegative multidrug. This page was last edited on 18 october 2019, at 16. This plant belongs to the rubiaceae family and is widely used in the african traditional medicine against malaria and malarialike symptoms.
Clinical trials with cochlospermum planchonii and nauclea pobeguinii showed that these two plant species can be considered as promising candidates for the development of herbal medicines in the treatment of uncomplicated falciparum malaria benoitvical et al. Therefore a perpetual search of new antimicrobial molecules is needed to face the challenge of microbial resistance. Abstractthe antimalarial activities of crude extracts and 17 fractions from the partition of 80%methanolic extracts of three plants the stem bark of croton mubango, the stem bark of nauclea pobeguinii and the leaves of pyrenacantha staudtii used as antimalarial remedies in the democratic republic of congo were studied both in vitro against plasmodium falciparum and in mice infected. It is known by the common names bilinga, aloma, badi, kusia and opepe. Ethnopharmacological uses of nauclea pobeguinii and nauclea diderrichii are also heavily documented whereas others species were largely left behind. However, no antimicrobial activity has been reported. Pdf antimalarial activity and toxicity evaluation of a.
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